RRAD Antibody

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ProSci
Product Code: PSI-43-008
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-008-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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A): 43-008 (0.5ug/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10ug protein in RIPA buffer) and compared with an HA-specific antibody. B): 43-008 (0.5ug/ml) staining of WT and KO lysates of Mouse Heart
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A): 43-008 (0.5ug/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10ug protein in RIPA buffer) and compared with an HA-specific antibody. B): 43-008 (0.5ug/ml) staining of WT and KO lysates of Mouse Heart

Documents

Further Information

Additional Names:
RRAD, Ras-related associated with diabetes, RAD, RAD1, REM3, GTP-binding protein RAD, OTTHUMP00000174805, RAS (RAD and GEM) like GTP binding 3, ras associated with diabetes
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Heart lysates and no 37kDa in the KO lysates (calculated MW of 33.2kDa according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged), a band of approx. 45kDa is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Recommended concentration: 0.5-2ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HRRSMPVDERDLQ
NCBI Gene ID #:
6236
NCBI Official Name:
Ras-related associated with diabetes
NCBI Official Symbol:
RRAD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 37 kDa
Protein Accession #:
NP_004156.1
Protein GI Number:
4759054
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Reported variants represent identical protein: NP_004156.1, NP_001122322.1
Swissprot #:
P55042