STK35 Antibody

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ProSci
Product Code: PSI-46-445
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-445-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-445 (2ug/ml) staining of nuclear HEK293 (A) and (1ug/ml) Human Breast cancer (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-445 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
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46-445 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
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46-445 (2ug/ml) staining of nuclear HEK293 (A) and (1ug/ml) Human Breast cancer (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-445 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue)
46-445 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

Documents

Further Information

Additional Names:
STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1kDa according to Human NP_543026.2, 59.5kDa according to Mouse NP_899085.3 and 59.2kDa according to XP_005672850.1 Pig XP_005672850.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: Goyal P et al, PLoS One. 2009 Sep 16;4(9):e6981. PMID: 19756140.Immunohistochemistry:In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Background References:
  • Vallenius T, Makela TP. Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. J Cell Sci. 2002 May 15;115(Pt 10):2067-73.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: ELETRMDQVTCAA
NCBI Gene ID #:
140901
NCBI Official Name:
serine/threonine kinase 35
NCBI Official Symbol:
STK35
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_543026.2
Protein GI Number:
20128768
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q8TDR2