ATG5 Antibody

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ProSci
Product Code: PSI-30-955
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-30-955-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATG5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 at 1 ug/ml.
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Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
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Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
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Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
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Antibody used in WB on Human HepG2 at 1 ug/ml.
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.

Documents

Further Information

Additional Names:
ATG5, APG5, APG5L, ASP, hAPG5, APG5-LIKE
Application Note:
ATG5 antibody can be used for detection of ATG5 by ELISA at 1:1562500. ATG5 antibody can be used for detection of ATG5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Background References:
  • Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATG5.
NCBI Gene ID #:
9474
NCBI Official Name:
autophagy related 5
NCBI Official Symbol:
ATG5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
NP_004840
Protein GI Number:
4757798
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Cancer ,Apoptosis
Swissprot #:
Q9H1Y0
User NOte:
Optimal dilutions for each application to be determined by the researcher.