Renalase Antibody

ProSci
Product Code: PSI-46-278
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-278-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-278 (1ug/ml) staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-278 (1ug/ml) staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
RNLS, renalase, C10orf59, chromosome 10 open reading frame 59 , HGNC:25641, FLJ11218 , hypothetical protein LOC55328, renalase, FAD-dependent amine oxidase, RENALASE
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38+35kDa bands observed in Human Heart lysates, 37kDa band observed in Mouse Heart lysates and approx 35kDa in Rat Heart lysates (calculated MW of 37.8 and 34.9 according to Human NP_001026879.2 and NP_060833.1 resp, of 33.3kDa according to Mouse NP_001161290.1 and of 35.0kDa according to Rat NP_001014189.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Background References:
  • Xu J, Li G, Wang P, Velazquez H, Yao X, Li Y, Wu Y, Peixoto A, Crowley S, Desir GV. Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure. J Clin Invest. 2005 May 2;115(5):1275-1280. Epub 2005 Apr 7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-VSIDNKKRNI
NCBI Gene ID #:
55328
NCBI Official Name:
renalase, FAD-dependent amine oxidase
NCBI Official Symbol:
RNLS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001026879.2, NP_060833.1
Protein GI Number:
72534708
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001026879.2 and NP_060833.1).
Swissprot #:
Q5VYX0