Orexin Receptor 2 Antibody

ProSci
Product Code: PSI-46-104
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-104-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-104 (1.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

46-104 (1.5ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
OTTHUMP00000039969, hypocretin (orexin) receptor 2, HCRTR2, OX2R, hypocretin receptor-2, orexin receptor 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 50.7kDa according to NP_001517.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Background References:
  • Spinazzi R, Rucinski M, Neri G, Malendowicz LK, Nussdorfer GG. Preproorexin and orexin receptors are expressed in cortisol-secreting adrenocortical adenomas, and orexins stimulate in vitro cortisol secretion and growth of tumor cells. J Clin Endocrinol Metab. 2005 Jun;90(6):3544-9. Epub 2005 Mar 29.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: SGTKLEDSPPCRN
NCBI Gene ID #:
3062
NCBI Official Name:
hypocretin (orexin) receptor 2
NCBI Official Symbol:
HCRTR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001517.1
Protein GI Number:
4557639
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O43614