PPP2R5E Antibody

ProSci
Product Code: PSI-46-212
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-212-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-212 (0.1ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-212 (0.1ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
PPP2R5E, protein phosphatase 2, regulatory subunit B (B56), epsilon isoform, PP2A, B subunit, B' epsilon isoform, PP2A, B subunit, R5 epsilon isoform, PP2A, B subunit, B56 epsilon isoform, PP2A, B subunit, PR61 epsilon isoform, Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon isoform, protein phosphatase 2, regulatory subunit B', epsilon isoform, PP2A, B subunit, R5 epsilon, epsilon isoform of regulatory subunit B56, protein phosphatase 2A, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 54.7kDa according to Human NP_001269108.1 and Mouse NP_036154.1 and 50.4kDa according to Rat NP_001100210.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem 1995 Nov 3;270(44):26123-8
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-LKRGLRRDGIIPT
NCBI Gene ID #:
5529
NCBI Official Name:
protein phosphatase 2, regulatory subunit B', epsilon isoform
NCBI Official Symbol:
PPP2R5E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006237.1
Protein GI Number:
5453956
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q16537