FAS Antibody

ProSci
Product Code: PSI-42-121
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-121-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-121 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-121 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
FAS, Fas (TNF receptor superfamily, member 6), ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, APO-1 cell surface antigen, CD95 antigen, Fas AMA, Fas antigen, OTTHUMP00000020045, OTTHUMP00000020046, OTTHUMP00000020051, OTTHUMP00000059646, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tumor necrosis factor receptor superfamily, member 6
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of cell line MOLT4 (calculated MW of 37.7kDa according to NP_000034.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75ug/ml.
Background References:
  • Blomberg J, Ruuth K, Jacobsson M, Höglund A, Nilsson JA, Lundgren E, Reduced FAS transcription in clones of U937 cells that have acquired resistance to Fas-induced apoptosis. FEBS J. 2009 Jan 276 (2): 497-508
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: KTCRKHRKENQGSH
NCBI Gene ID #:
355
NCBI Official Name:
Fas cell surface death receptor
NCBI Official Symbol:
FAS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000034.1, NP_690610.1, NP_690611.1
Protein GI Number:
4507583
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology ,Innate Immunity ,Apoptosis
SPECIFICITY:
This antibody is expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).
Swissprot #:
P25445