MSH5 Antibody

ProSci
Product Code: PSI-46-870
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-870-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-870 (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-870 (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
MSH5, mutS homolog 5 (E. coli) , XXbac-BCX40G17.5, DKFZp434C1615, G7N: MGC2939, MutSH5, NG23, mutS homolog 5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 38kDa band in Human Testis, Tonsil and Thymus lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9kDa according to the smallest reported isoform NP_002432.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis and Thymus. Recommended concentration: 2.5-3.75ug/ml.
Background References:
  • Neyton S, Lespinasse F, Lahaye F, Staccini P, Paquis-Flucklinger V, Santucci-Darmanin S. CRM1-dependent nuclear export and dimerization with hMSH5 contribute to the regulation of hMSH4 subcellular localization. Exp Cell Res. 2007 Oct 15;313(17):3680-93. Epub 2007 Aug 21.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: GDLHCEIRDQET
NCBI Gene ID #:
4439
NCBI Official Name:
mutS homolog 5
NCBI Official Symbol:
MSH5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079535.3 , NP_751897.1, NP_002432.1
Protein GI Number:
26638662
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Swissprot #:
O43196