TNFSF14 Antibody

ProSci
Product Code: PSI-42-126
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-126-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-126 (0.03ug/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-126 (0.03ug/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
TNFSF14, tumor necrosis factor (ligand) superfamily, member 14, CD258, HVEML, LIGHT, LTg, TR2, delta transmembrane LIGHT, herpesvirus entry mediator A, ligand for herpesvirus entry mediator, tumor necrosis factor ligand superfamily, member 14, tumor necrosis factor receptor-like 2, tumor necrosis factor superfamily member LIGHT
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 20-22kDa band observed in Human Cerebellum and Frontal Cortex Brain lysates (calculated MW of 22.4kDa according to NP_742011.1) Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membrane of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Cohavy O, Zhou J, Ware CF, Targan SR LIGHT is constitutively expressed on T and NK cells in the human gut and can be induced by CD2-mediated signaling J. Immunol. 2005 Jan 174 (2): 646-53
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HEVNPAAHLTGANS
NCBI Gene ID #:
8740
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 14
NCBI Official Symbol:
TNFSF14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003798.2, NP_742011.1
Protein GI Number:
25952144
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology ,Innate Immunity
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).
Swissprot #:
O43557