Renin Receptor Antibody

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ProSci
Product Code: PSI-46-280
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-280-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-280 (2ug/ml) of Mouse Kidney lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
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46-280 (2ug/ml) of Mouse Kidney lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ATP6AP2, ATP6IP2, ATPase, H+ transporting, lysosomal interacting protein 2, M8-9, APT6M8-9, ATP6M8-9, renin receptor, V-ATPase M8.9 subunit, ATPase membrane sector associated protein M8-9, vacuolar ATP synthase membrane sector associated protein M8-9, ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9, ELDF10, HT028, MGC99577, MRXE, MSTP009, XMRE, ATPase, H+ transporting, lysosomal interacting protein 2, embryonic liver differentiation factor 10
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Mouse Kidney lysates (calculated MW of 39.1kDa according to NP_081715.1). An additional band of 45kDa was also observed in some lysates, whch was completely blocked by the immunizing peptide. Recommended concentration:1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect.?2017 Jan;6(1):9-19, PMID: 27956412.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Background References:
  • Ludwig J, Kerscher S, Brandt U, Pfeiffer K, Getlawi F, Apps DK, Schagger H. Identification and characterization of a novel 9.2- kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules. J Biol Chem. 1998 May 1;273(18):10939-47.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-SIIYRMTNQKIRMD
NCBI Gene ID #:
10159
NCBI Official Name:
ATPase, H+ transporting, lysosomal accessory protein 2
NCBI Official Symbol:
ATP6AP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005756.2
Protein GI Number:
15011918
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O75787