APRIL Antibody
| Code | Size | Price |
|---|
| PSI-45-272-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ANP32B, SSP29, APRIL, PHAPI2, acidic (leucine-rich) nuclear phosphoprotein 32 family, member B, acidic protein rich in leucines, OTTHUMP00000063770
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Tonsil lysates, and approx 30kDa band observed in Rat and Pig Spleen lysates and in lysates of cell line NIH3T3 (calculated MW of 28.8kDa according to Human NP_006392.1, 31.1kDa according to Mouse NP_570959.1 and to Rat NP_571986.1 and 29.9kDa according to Pig XP_013849138.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows nuclear staining in lobular cells. Recommended concentration: 3-5ug/ml.
Background References:
- Zhu L, Perlaky L, Henning D, Valdez BC. Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family. Biochem Mol Biol Int. 1997 Aug;42(5):927-35.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KRKRETDDEGEDD
NCBI Gene ID #:
10541
NCBI Official Name:
acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
NCBI Official Symbol:
ANP32B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006392.1
Protein GI Number:
5454088
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).
Swissprot #:
Q92688