BCAP31 Antibody

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ProSci
Product Code: PSI-45-019
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-019-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-019 (0.1ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-019 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-019 (0.1ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-019 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
BCAP31, BAP31, DXS1357E, accessory protein BAP31, CDM, 6C6-AG, B-cell receptor-associated protein 31, CDM, 6C6 antigen, BCR-associated protein Bap31, OTTHUMP00000025978, p28 Bap31
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Placenta and Brain lysate (predicted MW of 28kDa according to NP_005736). Recommended for use at 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney and Brain (Cerebellum and Cortex). Recommended concentration: 3.75ug/ml.
Background References:
  • Adachi T, Schamel WW, Kim KM, Watanabe T, Becker B, Nielsen PJ, Reth M. The specificity of association of the IgD molecule with the accessory proteins BAP31/BAP29 lies in the IgD transmembrane sequence. EMBO J. 1996 Apr 1;15(7):1534-41.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QAAVDGPMDKKEE
NCBI Gene ID #:
10134
NCBI Official Name:
B-cell receptor-associated protein 31
NCBI Official Symbol:
BCAP31
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001132929.1, NP_005736.3
Protein GI Number:
32171186
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
This antibody is expected to recognise isoform a (NP_001132929.1) and isoform b (NP_005736.3; NP_001132913.1).
Swissprot #:
P51572