Cdc42-binding kinase alpha Antibody

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ProSci
Product Code: PSI-45-384
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-384-0.1mg0.1mg£626.00
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-384 (3.8ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-384 (3.8ug/ml) staining of paraffin embedded Human Cerebellum Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
CDC42BPA, MRCK, MRCKA, PK428, FLJ23347, KIAA0451, CDC42 binding protein kinase alpha (DMPK-like), ser-thr protein kinase PK428, myotonic dystrophy kinase-related CDC42-binding protein kinase alpha, Ser-Thr protein kinase related to the myotonic dystrophy protein kinase, DKFZp686L1738, CDC42 binding protein kinase beta
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 30kDa and 40kDa bands in Human Pancreas lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 186kDa according to NP_055641.3 and 196kDa according to NP_003598.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows cytoplasm staining in purkinje cells. Recommended concentration, 3-6ug/ml.
Background References:
  • Zhao Y, Loyer P, Li H, Valentine V, Kidd V, Kraft AS. Cloning and chromosomal location of a novel member of the myotonic dystrophy family of protein kinases. J Biol Chem. 1997 Apr 11;272(15):10013-20.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: SGEVRLRQLEQFI-C
NCBI Gene ID #:
8476
NCBI Official Name:
CDC42 binding protein kinase alpha (DMPK-like)
NCBI Official Symbol:
CDC42BPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055641.3, NP_003598.2
Protein GI Number:
30089960
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2).
Swissprot #:
Q5VT25