Cyclophilin A Antibody

ProSci
Product Code: PSI-45-442
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-442-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-442 (0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

45-442 (0.1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
PPIA, peptidylprolyl isomerase A (cyclophilin A) , HGNC:9253, CYPA, CYPH, MGC12404 , T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase, MGC117158, MGC23397, PPIase A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx.18kDa band observed in Human Lymph Node lysates (calculated MW 18.0kDa according to NP_066953.1). Recommended Concetration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Background References:
  • Berthoux L, Sebastian S, Sokolskaja E, Luban J. Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. Proc Natl Acad Sci USA. 2005 Oct 11;102(41):14849-53. Epub 2005 Oct 3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ERFGSRNGKTSKK
NCBI Gene ID #:
5478
NCBI Official Name:
peptidylprolyl isomerase A (cyclophilin A)
NCBI Official Symbol:
PPIA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_066953.1
Protein GI Number:
10863927
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
This product is expected to also recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).
Swissprot #:
P62937