CYP7B1 Antibody

ProSci
Product Code: PSI-45-449
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-449-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-449 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

45-449 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Further Information

Additional Names:
CYP7B1, CP7B, cytochrome P450, family 7, subfamily B, polypeptide 1, oxysterol 7alpha-hydroxylase, cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1, CBAS3, SPG5A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments in Human Liver, Kidney and Prostate lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Breast shows textured cytoplasm staining in ductal epithelial cells. Recommended concentration, 3-6ug/ml.
Background References:
  • Wu Z, Martin KO, Javitt NB, Chiang JY. Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1. J Lipid Res. 1999 Dec;40(12):2195-203.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Cow
Immunogen:
The immunogen for this antibody is: C-YPDSDVLFRYKVKS
NCBI Gene ID #:
9420
NCBI Official Name:
cytochrome P450, family 7, subfamily B, polypeptide 1
NCBI Official Symbol:
CYP7B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004811.1
Protein GI Number:
4758104
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O75881