Cytochrome B Reductase 1 Antibody
| Code | Size | Price |
|---|
| PSI-45-452-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1?g/ml antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfully used in WB on Human in PMID: 24894955, 21973163 and 18830567.Immunohistochemistry:In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 2.5ug/ml.
Background References:
- Atanasova BD, Li AC, Bjarnason I, Tzatchev KN, Simpson RJ. Duodenal ascorbate and ferric reductase in human iron deficiency. Am J Clin Nutr. 2005 Jan;81(1):130-3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CRNLALDEAGQRSTM
NCBI Gene ID #:
79901
NCBI Official Name:
cytochrome b reductase 1
NCBI Official Symbol:
CYBRD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079119.3
Protein GI Number:
188536036
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_079119.3) only.
Swissprot #:
Q53TN4