DDAH2 Antibody

ProSci
Product Code: PSI-45-469
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-469-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-469 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

45-469 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
DDAH2, dimethylarginine dimethylaminohydrolase 2, DDAH, G6a, NG30, DDAHII, dimethylarginine dimethylaminohydrolase II, NG-dimethylarginine dimethylamino hydrolase homolog, OTTHUMP00000062666, DADB-110M10.5, OTTHUMP00000029307, OTTHUMP00000174406, alternative name: NG30, G6a, dimethylarginine dimethylaminohydrolase II
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Lung lysates (calculated MW of 29.6kDa according to NP_039268.1). Recommended concentration: 0.1-0.3?g/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunofluorescence:Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10ug/ml.Flow Cytometry:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Background References:
  • Leiper JM, Santa Maria J, Chubb A, MacAllister RJ, Charles IG, Whitley GS, Vallance P. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 1999 Oct 1;343 Pt 1:209-14
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: SSLCLVLSTRPHS
NCBI Gene ID #:
23564
NCBI Official Name:
dimethylarginine dimethylaminohydrolase 2
NCBI Official Symbol:
DDAH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_039268.1
Protein GI Number:
7524354
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
.
Swissprot #:
O95865