GTF2IRD1 Antibody
| Code | Size | Price |
|---|
| PSI-45-714-0.1mg | 0.1mg | £565.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
GTF2IRD1, GTF2I repeat domain containing 1 , HGNC:4661, CREAM1, GTF3, MUSTRD1, RBAP2, WBSCR11, WBSCR12, hMusTRD1alpha1 , GTF2I repeat domain-containing 1, Williams-Beuren syndrome chromosome region 11, general transcription factor 3, muscle TFII-I repeat
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 30kDa and 40kDa in Human Heart lysate after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 106kDa according to NP_057412.1 and NP_005676.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Tassabehji M, Hammond P, Karmiloff-Smith A, Thompson P, Thorgeirsson SS, Durkin ME, Popescu NC, Hutton T, Metcalfe K, Rucka A, Stewart H, Read AP, Maconochie M. GTF2IRD1 in Craniofacial Development of Humans and Mice. Science. 2005 Nov 18;310(5751):1184-7. Epub 2005 Nov 3.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-NKFTKDTTKLEPAS
NCBI Gene ID #:
9569
NCBI Official Name:
GTF2I repeat domain containing 1
NCBI Official Symbol:
GTF2IRD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057412.1, NP_005676.2
Protein GI Number:
7705387
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_057412.1 and NP_005676.2).
Swissprot #:
Q9UHL9