Insulysin Antibody
| Code | Size | Price |
|---|
| PSI-45-776-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
insulinase, insulysin, insulin-degrading enzyme , FLJ35968, INSULYSIN
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5ug/ml.
Background References:
- Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiol Aging. 2007 Jun;28(6):824-30. Epub 2006 Jun 12.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QQYNFDRDNTE
NCBI Gene ID #:
3416
NCBI Official Name:
insulin-degrading enzyme
NCBI Official Symbol:
IDE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004960.2, NP_001159418.1, NP_001309722.1, NP_001309723.1, NP_001309724.1, NP_001309726.1
Protein GI Number:
4826770
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize all reported isoforms (NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1
Swissprot #:
P14735