Klotho Antibody

ProSci
Product Code: PSI-45-810
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-810-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-810 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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45-810 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Further Information

Additional Names:
KL, klotho
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Brain, Duodenum, Placenta and Prostate lysates gave no specific signal but low background at antibody concentration up to 1ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining in luminal epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration: 4-6ug/ml.
Background References:
  • Kurosu H, Yamamoto M, Clark JD, Pastor JV, Nandi A, Gurnani P, McGuinness OP, Chikuda H, Yamaguchi M, Kawaguchi H, Shimomura I, Takayama Y, Herz J, Kahn CR, Rosenblatt KP, Kuro-o M. Suppression of ageing in mice by the hormone Klotho. Science. 2005 Sep 16;309(5742):1829-33. Epub 2005 Aug 25.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-KRDDAKYMYYLKK
NCBI Gene ID #:
9365
NCBI Official Name:
klotho
NCBI Official Symbol:
KL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004786.2, NP_710150.1
Protein GI Number:
24497614
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_004786.2; NP_710150.1).
Swissprot #:
Q9UEF7