MKK3 Antibody

Contact us
ProSci
Product Code: PSI-79-171
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-171-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

1 / 2
Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189).
2 / 2
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Phospho-Ser189).
❮❯

Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Phospho-Ser189).

Documents

Further Information

Additional Names:
MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Background References:
  • Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MKK3 (Phospho-Ser189) antibody was raised against a peptide sequence around phosphorylation site of serine 189 (V-D-S (p) -V-A) derived from Human MKK3.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5606
NCBI Official Name:
mitogen-activated protein kinase kinase 3
NCBI Official Symbol:
MAP2K3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
NP_002747.2
Protein GI Number:
21618347
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of MKK3 only when phosphorylated at serine189.
Swissprot #:
P46734