NSF Antibody
| Code | Size | Price |
|---|
| PSI-50-183-0.1mL | 0.1mL | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
NSF antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Documents
Further Information
Application Note:
The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Background:
NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).
Background References:
- Schweizer FE, Dresbach T, DeBello WM, O?Connor V, Augustine G, Betz H (1998) Regulation of neurotransmitter release kinetics by NSF. Science 279(5354):1203-1206.
- Matveeva EA, Whitehart SW, Vanaman T, Slevin J (2001) Phosphorylation of the N-ethylmaleimide sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes. J Biol.Chem. 276(15): 12174-12181.
- Whitehart SW, Matveeva EA (2004) Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor. J Struct. Biol.146(1-2): 32-43.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH).
NCBI Gene ID #:
60355
NCBI Official Name:
N-ethylmaleimide-sensitive factor
NCBI Official Symbol:
Nsf
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75
Protein Accession #:
Q9QUL6
Protein GI Number:
81917630
Purification:
Affinity Purified
Swissprot #:
Q9QUL6
User NOte:
Optimal dilutions for each application to be determined by the researcher.