TAF8 Antibody

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ProSci
Product Code: PSI-42-253
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-253-0.1mg0.1mg£505.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Documents

Further Information

Additional Names:
TAF8, TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa, 43, FLJ32821, II, TAF, TAFII43, TBN, OTTHUMP00000016392, TAF(II)43, TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50 kD, TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa, TBP-associated factor 8, TBP-associated factor TAFII43, TBP-associated factor, RNA polymerase II, 43 kD, taube nuss homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 38kDa band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 34.3kDa according to NP_612639.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Guermah M, Ge K, Chiang CM, Roeder RG, The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis. Molecular cell 2003 Oct 12 (4): 991-1001.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: KSYCEHTARTQPT
NCBI Gene ID #:
129685
NCBI Official Name:
TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
NCBI Official Symbol:
TAF8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_612639.2
Protein GI Number:
104485446
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription
Swissprot #:
Q7Z7C8