GRIA4 Antibody

ProSci
Product Code: PSI-46-724
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-724-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-724 (2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-724 (2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
GRIA4 , glutamate receptor, ionotrophic, AMPA 4 , GLUR4, GLUR4C, GLURD, AMPA-selective glutamate receptor 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx.100kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2kDa according to NP_001070711.2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10ug/ml.
Background References:
  • Earnshaw BA, Bressloff PC. Biophysical model of AMPA receptor trafficking and its regulation during long-term potentiation/long-term depression. J Neurosci. 2006 Nov 22;26(47):12362-73.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-KKLDQREYPGSETP
NCBI Gene ID #:
2893
NCBI Official Name:
glutamate receptor, ionotropic, AMPA 4
NCBI Official Symbol:
GRIA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000820.3, NP_001070711.2, NP_001070712.1
Protein GI Number:
164419734
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Neuroscience
SPECIFICITY:
This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.
Swissprot #:
P48058