LHCGR Antibody
| Code | Size | Price |
|---|
| PSI-45-830-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
LHCGR, luteinizing hormone/choriogonadotropin receptor , LCGR, LGR2, LHR, hLHR , luteinizing hormone receptor, lutropin/choriogonadotropin receptor, LH receptor, CG receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 35kDa band in K562 cell lysates after 0.1ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we cannot currently find an explanation in the literature for this band, given the calculated size of 78.6kDa according to NP_000224.2.Immunofluorescence: Strong expression of the protein seen in the plasma membranes of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Background References:
- Bukovsky A, Indrapichate K, Fujiwara H, Cekanova M, Ayala ME, Dominguez R, Caudle MR, Wimalsena J, Elder RF, Copas P, Foster JS, Fernando RI, Henley DC, Upadhyaya NB. Multiple luteinizing hormone receptor (LHR) protein variants, interspecies reactivity of anti-LHR mAb clone 3B5, subcellular localization of LHR in human placenta, pelvic floor and brain, and possible role for LHR in the development of abnormal pregnancy, pelvic floor disorders and Alzheimer's disease. Reprod Biol Endocrinol. 2003 Jun 3;1:46.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: CQGTALLDKTRYTE
NCBI Gene ID #:
3973
NCBI Official Name:
luteinizing hormone/choriogonadotropin receptor
NCBI Official Symbol:
LHCGR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000224.2
Protein GI Number:
106067657
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
P22888