GRO-B Antibody

Product Code: PSI-XP-5148

Supplier: ProSci

Code	Size	Price

PSI-XP-5148-0.1mg

0.1mg

£524.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Storage:

GRO-B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

Images

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To detect Human GRO-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human GRO-beta (XP-5148Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO-beta.

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To detect human GRO-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 1.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

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Documents

Further Information

Additional Names:

GRO2, GROb, MIP2, MIP2A, SCYB2, MGSA-b, MIP-2a, CINC-2a, GRO2, GROB, C-X-C motif chemokine 2, Growth-regulated protein beta, Gro-beta

Application Note:

ELISA:

To detect hGRO beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO beta.

Sandwich: To detect hGRO-β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO-β (XP-5148Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-β.

Western Blot:

To detect hGRO beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Concentration:

batch dependent

Conjugate:

Unconjugated

Immunogen:

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO beta (human GRO-beta).

NCBI Gene ID #:

2920

NCBI Official Name:

chemokine (C-X-C motif) ligand 2

NCBI Official Symbol:

CXCL2

NCBI Organism:

Homo sapiens

Physical State:

Lyophilized

Protein Accession #:

P19875

Protein GI Number:

127085

Purification:

Anti-hGRO beta specific antibody was purified by affinity chromatography employing immobilized hGRO beta matrix.

Research Area:

Chemokines & Cytokines,Signal Transduction,Homeostasis,Antibody Pairs

Swissprot #:

P19875

User NOte:

Centrifuge vial prior to opening.