IL-15 Antibody

ProSci
Product Code: PSI-XP-5169Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5169Bt-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
IL-15 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-15 (XP-5169) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hIL-15.

To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-15 (XP-5169) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hIL-15.

Further Information

Additional Names:
CD122, IL15RB, P70-75Interleukin-15IL-15
Application Note:
ELISA:

Sandwich:
To detect hIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-15 (XP-5169) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15.

Western Blot:

To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-15 (human Interleukin-15).
NCBI Gene ID #:
3560
NCBI Official Name:
interleukin 2 receptor, beta
NCBI Official Symbol:
IL2RB
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P40933-2
Protein GI Number:
729830
Purification:
Anti-hIL-15 specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P40933-2
User NOte:
Centrifuge vial prior to opening.