IFN-gamma Antibody
Code | Size | Price |
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PSI-XP-5156-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IFN-gamma antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fre
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Further Information
Additional Names:
Ifg, IFN-gInterferon gammaIFN-gamma
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIFN-gamma (50 pg/mL), a concentration of 0.04 - 0.06 ng/mL of this antibody is required.
ELISA:
To detect mIFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIFN-gamma.
Sandwich:
To detect mIFN-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IFN-γ (XP-5156Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIFN-γ.
Western Blot:
To detect mIFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mIFN-gamma (50 pg/mL), a concentration of 0.04 - 0.06 ng/mL of this antibody is required.
ELISA:
To detect mIFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIFN-gamma.
Sandwich:
To detect mIFN-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IFN-γ (XP-5156Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIFN-γ.
Western Blot:
To detect mIFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIFN-gamma (murine Interferon-gamma).
NCBI Gene ID #:
15978
NCBI Official Name:
interferon gamma
NCBI Official Symbol:
Ifng
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P01580
Protein GI Number:
124480
Purification:
Anti-mIFN-gamma specific antibody was purified by affinity chromatography employing immobilized mIFN-gamma matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P01580
User NOte:
Centrifuge vial prior to opening.