IL-13 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5167-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IL-13 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
P600, IL-13, NC30, Interleukin-13IL-13
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/mL), a concentration of 0.12 - 0.15 μg/mL of this antibody is required.
ELISA:
To detect hIL-13 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13.
Sandwich
To detect hIL-13 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-13 (XP-5167Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-13.
Western Blot:
To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/mL), a concentration of 0.12 - 0.15 μg/mL of this antibody is required.
ELISA:
To detect hIL-13 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13.
Sandwich
To detect hIL-13 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-13 (XP-5167Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-13.
Western Blot:
To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-13 (human Interleukin-13).
NCBI Gene ID #:
3596
NCBI Official Name:
interleukin 13
NCBI Official Symbol:
IL13
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P35225
Protein GI Number:
239938644
Purification:
Anti-hIL-13 specific antibody was purified by affinity chromatography employing immobilized hIL-13 matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P35225
User NOte:
Centrifuge vial prior to opening.