PRMT5 Antibody
| Code | Size | Price |
|---|
| PSI-29-204-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PRMT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
PRMT5, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
Application Note:
PRMT5 antibody can be used for detection of PRMT5 by ELISA at 1:62500. PRMT5 antibody can be used for detection of PRMT5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.
Background References:
- Azzouz, T.N., (2005) J. Biol. Chem. 280 (41), 34435-34440.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT5.
NCBI Gene ID #:
10419
NCBI Official Name:
protein arginine methyltransferase 5
NCBI Official Symbol:
PRMT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa, 73 kDa
Protein Accession #:
NP_001034708
Protein GI Number:
88900507
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Cancer ,Homeostasis
Swissprot #:
O14744
User NOte:
Optimal dilutions for each application to be determined by the researcher.