Granzyme B (B18.1) Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-XA-1005-0.1mg | 0.1mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Granzyme B antibody can be stored at 4˚C, stable for one year.
Documents
Further Information
Application Note:
Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, immunocytochemistry, immunohistochemistry (frozen sections, paraffin sections), immunoprecipitation and western blot. Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg of FITC- labeled B18.1 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 μg/mL of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
Background References:
- Granzyme B and perforin can be used as predictive markers of acute rejection in heart transplantation: S. Legros-Maida, et al.; Eur. J. Immunol. 24, 229 (1994).
- Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients: M.V. Clement, et al.; Transplantation 57, 322 (1994).
- Granzyme B and perforin lytic proteins are expressed in CD34+ peripheral blood progenitor cells mobilized by chemotherapy and granulocyte colony-stimulating factor: C. Berthou, et al.; Blood 86, 3500 (1995).
- Expansion of a peripheral blood perforin+ CD8+ T-cell subset in long- term surviving lung transplanted patients: C. Berthou, et al.; Transplant. Proc. 28, 1964 (1996).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Granzyme B (monoclonal B18.1) antibody was raised against recombinant human granzyme B, amino acids 7-227.
NCBI Gene ID #:
3002
NCBI Official Name:
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
NCBI Official Symbol:
GZMB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Granzyme B (monoclonal B18.1) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Apoptosis
Swissprot #:
P10144
User NOte:
Optimal dilutions for each application to be determined by the researcher.