WRNIP1 Antibody
| Code | Size | Price |
|---|
| PSI-46-600-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
WHIP, Werner helicase interacting protein, FLJ22526, putative helicase RUVBL, Werner helicase interacting protein 1, RP11-420G6.2, bA420G6.2,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 72.1kDa according to NP_064520.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bish RA, Myers MP., J Biol Chem. 2007 Aug 10;282(32):23184-93; PMID: 17550899). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT. Recommended concentration, 4-6ug/ml.
Background References:
- Kawabe Yi, Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo SJ, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T. A novel protein interacts with the Werner's syndrome gene product physically and functionally. J Biol Chem. 2001 Jun 8;276(23):20364-9. Epub 2001 Apr 11.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: EELRGVDFFKQRRC
NCBI Gene ID #:
56897
NCBI Official Name:
Werner helicase interacting protein 1
NCBI Official Symbol:
WRNIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_064520.2, NP_569079.1
Protein GI Number:
18426904
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_064520.2) and isoform 2 (NP_569079.1).
Swissprot #:
Q96S55