Cardiotrophin-1 Antibody

ProSci
Product Code: PSI-XP-5113
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5113-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
Cardiotrophin-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeat

Images

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To detect hCT-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human CT-1 (XP-5113Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCT-1.
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This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

To detect hCT-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human CT-1 (XP-5113Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCT-1.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
CT1, CT-1Cardiotrophin-1CT-1
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hCardiotrophin 1 (15 ng/mL), a concentration of 0.25 - 0.40 μg/mL of this antibody is required.

ELISA:

To detect hCardiotrophin 1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hCardiotrophin 1.

Western Blot:
To detect hCardiotrophin 1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCardiotrophin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCardiotrophin 1 (human Cardiotrophin-1).
NCBI Gene ID #:
1489
NCBI Official Name:
cardiotrophin 1
NCBI Official Symbol:
CTF1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q16619
Protein GI Number:
2498265
Purification:
Anti-hCardiotrophin 1 specific antibody was purified by affinity chromatography employing immobilized hCardiotrophin 1 matrix.
Research Area:
Immunology,Antibody Pairs, Functional Assays
Swissprot #:
Q16619
User NOte:
Centrifuge vial prior to opening.