IFN-gamma Antibody
| Code | Size | Price |
|---|
| PSI-XP-5155Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IFN-gamma antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fre
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Further Information
Additional Names:
IFG, IFIInterferon gamma, Immune interferon, IFN-gamma
Application Note:
ELISA:
Sandwich:
To detect hIFN-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IFN-γ (XP-5155) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIFN-γ.
Western Blot:
To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hIFN-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IFN-γ (XP-5155) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIFN-γ.
Western Blot:
To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIFN-gamma (human Interferon-gamma).
NCBI Gene ID #:
3458
NCBI Official Name:
interferon, gamma
NCBI Official Symbol:
IFNG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01579
Protein GI Number:
124479
Purification:
Anti-hIFN-gamma specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P01579
User NOte:
Centrifuge vial prior to opening.