Betacellulin Antibody

ProSci
Product Code: PSI-38-180
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-180-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Betacellulin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated

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To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human Betacellulin (38-179) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hBetacellulin.

To detect hBetacellulin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human Betacellulin (38-179) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hBetacellulin.

Further Information

Additional Names:
ProbetacellulinBTC
Application Note:
ELISA:

Sandwich:
To detect hBetacellulin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Betacellulin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Western Blot:
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG).
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Human Betacellulin specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
685
NCBI Official Name:
betacellulin
NCBI Official Symbol:
BTC
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P35070
Protein GI Number:
461653
Research Area:
Antibody Pairs
Swissprot #:
P35070
User NOte:
Centrifuge vial prior to opening.