RNF25 Antibody

ProSci
Product Code: PSI-29-853
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-29-853-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store RNF25 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in IHC on Human Skin.
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Antibody used in IHC on Human Skin.

Further Information

Additional Names:
RNF25, AO7, FLJ13906
Application Note:
RNF25 antibody can be used for detection of RNF25 by ELISA at 1:62500. RNF25 antibody can be used for detection of RNF25 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
RNF25 contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.
Background References:
  • Asamitsu, K., (2003) J. Biol. Chem. 278 (29), 26879-26887.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF25.
NCBI Gene ID #:
64320
NCBI Official Name:
ring finger protein 25
NCBI Official Symbol:
RNF25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
NP_071898
Protein GI Number:
34878787
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Apoptosis ,Homeostasis
Swissprot #:
Q96BH1
User NOte:
Optimal dilutions for each application to be determined by the researcher.