ASK1 Antibody

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ProSci
Product Code: PSI-79-329
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-329-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MDA-MB- 435 cells using ASK1 (Ab-83).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Ab-83).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using ASK1 (Ab-83).
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Western blot analysis of lysed extracts from MDA-MB- 435 cells using ASK1 (Ab-83).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Ab-83).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using ASK1 (Ab-83).

Documents

Further Information

Additional Names:
ASK1, MEKK5, MAPKKK5, HER3, ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Background References:
  • Mabuchi S, et al. (2004) Endocrinology. 145 (1): 49-58. Yuan ZQ, et al. (2003) J Biol Chem. 278 (26): 23432-23440. Kim AH, et al. (2001) Mol Cell Biol. 21 (3): 893-901.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ASK1 (Ab-83) antibody was raised against a peptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1.
NCBI Gene ID #:
4217
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol:
MAP3K5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
155 kDa
Protein Accession #:
NP_005914.1
Protein GI Number:
5174547
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total ASK1 protein.
Swissprot #:
Q99683