BCL-2 Antibody

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ProSci
Product Code: PSI-79-299
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-299-0.1mg0.1mg£481.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MCF cells using BCL-2 (Ab-70).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-70).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-70).
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Western blot analysis of lysed extracts from MCF cells using BCL-2 (Ab-70).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-70).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-70).

Documents

Further Information

Additional Names:
Bcl-2, PPP1R50, PKR, PRKR, BCL2
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
BCL-2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Background References:
  • Muller IM, et al. (2005) Mol Pharmacol. Jin Z, Gao F, et al. J Biol Chem 2004 Sep 17; 279 (38): 40209-19. Kumar Biswas S, et al. Mol Cancer Ther 2004 Mar; 3 (3): 327-34. Huang ST, et al. (2002) FASEB J; 16 (8): 825-32. Yamamoto K, et al. (1999) Mol Cell Biol; 19 (12): 8469-78.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BCL-2 (Ab-70) antibody was raised against a peptide sequence around aa.68~72 (R-T-S-P-L) derived from Human BCL-2.
NCBI Gene ID #:
596
NCBI Official Name:
B-cell CLL/lymphoma 2
NCBI Official Symbol:
BCL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
NP_000624.2
Protein GI Number:
72198189
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total BCL-2 protein.
Swissprot #:
P10415