Cortactin Antibody
| Code | Size | Price |
|---|
| PSI-79-489-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
EMS1, PKB, RAC, Amplaxin, CTTN, EMS1, SRC8
Application Note:
Western Blot: 1:500~1:1000
Background:
Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
Background References:
- Luo C, et al. (2006) J Biol Chem ; 281 (40): 30081-30093Head JA, et al. (2003) Mol Biol Cell ;14 (8): 3216-3229Li Y, et al. (2000) J Biol Chem ; 275 (47): 37187-37193Kinley AW, et al. (2003) Curr Biol ; 13 (5): 384-393
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Cortactin (Phospho-Tyr466) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y (p) -E-T) derived from Human CORTACTIN.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2017
NCBI Official Name:
cortactin
NCBI Official Symbol:
CTTN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
NP_005222.2
Protein GI Number:
20357552
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.
Swissprot #:
Q14247