Histone H3.1 Antibody

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ProSci
Product Code: PSI-79-555
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-555-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells using Histone H3.1 (Ab-10).
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Immunofluorescence staining of methanol-fixed HeLa cells using Histone H3.1 (Ab-10).
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Western blot analysis of lysed extracts from 293 cells using Histone H3.1 (Ab-10).
Immunofluorescence staining of methanol-fixed HeLa cells using Histone H3.1 (Ab-10).

Documents

Further Information

Additional Names:
H3/b, H3FB, H3/a, H3/c, H3/d, H3/f, H3/h
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background References:
  • Workman, J. L. and Kingston, R. E. (1998) Annu Rev Biochem 67, 545-79. Hansen, J. C. et al. (1998) Biochemistry 37, 17637-41. Strahl, B. D. and Allis, C. D. (2000) Nature 403, 41-5. Cheung, P. et al. (2000) Cell 103, 263-71.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Histone H3.1 (Ab-10) antibody was raised against a peptide sequence around aa.8~12 (R-K-S-T-G) derived from Human Histone H3.1.
NCBI Gene ID #:
8351
NCBI Official Name:
histone cluster 1, H3d
NCBI Official Symbol:
HIST1H3D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
NP_003521.2
Protein GI Number:
21071021
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Histone H3.1 protein.
Swissprot #:
P68431