MARCKS Antibody

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ProSci
Product Code: PSI-79-486
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-486-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using MARCKS (Phospho-Ser162).
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Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS (Phospho-Ser162).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using MARCKS (Phospho-Ser162).
Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS (Phospho-Ser162).

Documents

Further Information

Additional Names:
MACS, 80K-L, PKCSL, PRKCSL, TSFP1, MACS, MARCS, Protein kinase C substrate
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Background References:
  • Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102 (35): 12407-12412Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280 (3): 605-609Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359 (2): 151-159
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4082
NCBI Official Name:
myristoylated alanine-rich protein kinase C substrate
NCBI Official Symbol:
MARCKS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
NP_002347.5
Protein GI Number:
153070260
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Neuroscience
SPECIFICITY:
This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
Swissprot #:
P29966