MyoD Antibody

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ProSci
Product Code: PSI-79-443
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-443-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MyoD (Phospho-Ser200).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MyoD (Phospho-Ser200).

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Further Information

Additional Names:
PUM, MYF3, MYOD, bHLHc1, IBP3, MYOD1
Application Note:
Western Blot: 1:500~1:1000
Background:
MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Background References:
  • Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24 (4): 1809-1821. Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19 (4): 3167-3176.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MyoD (Phospho-Ser200) antibody was raised against a peptide sequence around phosphorylation site of serine 200 (A-S-S (p) -P-R) derived from Human MyoD.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4654
NCBI Official Name:
myogenic differentiation 1
NCBI Official Symbol:
MYOD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
NP_002469.2
Protein GI Number:
23111009
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of MyoD only when phosphorylated at serine 200.
Swissprot #:
P15172