AURKB Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-114-0.1mL | 0.1mL | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 13E8D3
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
AURKB monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora 1, AIM-1, AIK2, AurB, IPL1, PPP1R48, aurkb-sv1, aurkb-sv2
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39 kDa),localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates Chromosomal segregation during mitosis and meiosis.It may regulates several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis.Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts arepresent at high levels in human thymus and fetal liver.ARK-2 protein levels are maximal during both S and G2/M phases
Background References:
- Song J et al. Immunity 22:621-31 (2005).
- Kapoor P et al. Mol Cell Biol 25:4934-45 (2005).
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant AURKB expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
9212
NCBI Official Name:
aurora kinase B
NCBI Official Symbol:
AURKB
NCBI Organism:
Homo sapiens
Protein Accession #:
Q96GD4
Protein GI Number:
317373473
Research Area:
Signal Transduction
Swissprot #:
Q96GD4
User NOte:
Optimal dilutions for each application to be determined by the researcher.