p53 Antibody
| Code | Size | Price |
|---|
| PSI-79-447-0.1mg | 0.1mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
P53, BCC7, LFS1, TRP53, HSP27, HSP28, Antigen NY-CO-13, Phosphoprotein p53, TP53, Tumor suppressor p53
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.
Background References:
- Lin T, et al. (2005) Nat Cell Biol; 7 (2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23): 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38): 39584-92.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p53 (Phospho-Ser33) antibody was raised against a peptide sequence around phosphorylation site of serine 33 (V-L-S (p) -P-L) derived from Human p53.
Modifications:
Phospho-Specific
NCBI Gene ID #:
7157
NCBI Official Name:
tumor protein p53
NCBI Official Symbol:
TP53
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
NP_000537.3
Protein GI Number:
120407068
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer,Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of p53 only when phosphorylated at serine 33.
Swissprot #:
P04637