AMACR Monoclonal Antibody

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ProSci
Product Code: PSI-32-107
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-107-0.1mL0.1mL£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2A10F3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
AMACR monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using AMACR monoclonal antibody against truncated AMACR - His recombinant protein and Jurkat cell lysate.
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Western blot analysis using AMACR monoclonal antibody against truncated AMACR - His recombinant protein and Jurkat cell lysate.

Documents

Further Information

Additional Names:
2-methylacyl-CoA racemaseRM, RACE, CBAS4, AMACRD
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
Background References:
  • Chen Q. Watson JT. Marengo SR. et al. Cancer Lett. 2006, Dec 8, 244 (2):274-88.Epub 2006 Feb 23.
  • Epstein JI. Herawi M. J Urol. 2006, Mar, 175 (3 Pt 1):820-34. Review.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
23600
NCBI Official Name:
alpha-methylacyl-CoA racemase
NCBI Official Symbol:
AMACR
NCBI Organism:
Homo sapiens
Protein Accession #:
Q9UHK6
Protein GI Number:
313104070
Research Area:
Signal Transduction ,Neuroscience
Swissprot #:
Q9UHK6
User NOte:
Optimal dilutions for each application to be determined by the researcher.