Smad2 Antibody

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ProSci
Product Code: PSI-79-500
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-500-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Thr220).
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Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Thr220).

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Further Information

Additional Names:
JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, ERBB, ERBB1, HER1, Mad-related protein 2, Mothers against DPP homolog 2
Application Note:
Western Blot: 1:500~1:1000
Background:
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Background References:
  • Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15 (2): 420?434. Rebecca A. Randall et al. (2004) Mol Cell Biol. February; 24 (3): 1106?1121. Kimberly A Brown et al. (2004) Breast Cancer Res. ; 6 (3): R215?R231.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Smad2 (Phospho-Thr220) antibody was raised against a peptide sequence around phosphorylation site of threonine 220 (P-E-T (p) -P-P) derived from Human Smad2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4087
NCBI Official Name:
SMAD family member 2
NCBI Official Symbol:
SMAD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_001003652.1
Protein GI Number:
51173730
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.
Swissprot #:
Q15796