GNAI3 Antibody

ProSci
Product Code: PSI-XW-7212
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XW-7212-0.05mg0.05mg£801.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Immunocytochemistry (ICC)
Storage:
GNAI3 antibody can be stored at 4˚C for short term (weeks). Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot detection of endogenous Gai3 protein in normal rat kidney cell line. Anti-Gai3 IgY dilution: 1:1000; goat anti-IgY Fc-HRP: 1:10,000.

Western blot detection of endogenous Gai3 protein in normal rat kidney cell line. Anti-Gai3 IgY dilution: 1:1000; goat anti-IgY Fc-HRP: 1:10,000.

Further Information

Additional Names:
Gai3
Application Note:
Gai3 antibody can be used for the detection of Gai3 by ICC, may also work for IHC and Wetern blot.
Background:
GNαi3, Guanine nucleotide binding protein (G protein), α inhibiting activity polypeptide 3. Gαi3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase following its G-protein coupled receptors (GPCR) such as a2-adrenergic receptors are activated. Gαi3 can be inactivated by pertussis toxin.
Background References:
  • Miotti,S., Bagnoli,M., Tomassetti,A., Colnaghi,M. I. and Canevari,S. Interaction of folate receptor with signaling molecules lyn and G(alpha)(i-3) in detergent-resistant complexes from the ovary carcinoma cell line IGROV1 J. Cell. Sci. 113 Pt 2, 349-357 (2000).
Buffer:
Phosphate-Buffered Saline. No preservatives added.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
C-terminal peptide (KNNLKECGLY)
NCBI Gene ID #:
2773
NCBI Official Name:
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
NCBI Official Symbol:
GNAI3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40.4 kDa (calculated)
Protein Accession #:
NP_006487.1
Protein GI Number:
5729850
Purification:
Antigen affinity-purified
Research Area:
Signal Transduction, Autophagy
Swissprot #:
P08754
User NOte:
Optimal dilutions for each application to be determined by the researcher.