FXR1 Antibody

Contact us
ProSci
Product Code: PSI-45-058
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-058-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 4
45-058 (1ug/ml) staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
2 / 4
3 / 4
4 / 4
45-058 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
❮❯

45-058 (1ug/ml) staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-058 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Documents

Further Information

Additional Names:
FXR1, fragile X mental retardation, autosomal homolog 1 , fragile X mental retardation-related protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration. 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Coon H, Matsunami N, Stevens J, Miller J, Pingree C, Camp NJ, Thomas A, Krasny L, Lainhart J, Leppert MF, McMahon W. Evidence for linkage on chromosome 3q25-27 in a large autism extended pedigree. Hum Hered. 2005;60(4):220-6. Epub 2006 Jan 2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RIEGDNENKLPRED
NCBI Gene ID #:
8087
NCBI Official Name:
fragile X mental retardation, autosomal homolog 1
NCBI Official Symbol:
FXR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005078.2, NP_001013456.1, NP_001013457.1
Protein GI Number:
61835148
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).
Swissprot #:
P51114