LIME1 Antibody

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ProSci
Product Code: PSI-45-085
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-085-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-085 staining (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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45-085 staining (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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Further Information

Additional Names:
Lime1, Lck interacting transmembrane adaptor 1, 2810038K19Rik, LIME, RIKEN cDNA 2810038K19 gene, RP23-60M5.4, OTTMUSP00000018005
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 32kDa band observed in Mouse Heart lysates (predicted MW of 31kDa according to NP_076173.1). Recommended for use at 0.5-2ug/ml.
Background References:
  • Hur EM, Son M, Lee OH, Choi YB, Park C, Lee H, Yun Y. LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation. J Exp Med. 2003 Nov 17;198(10):1463-73. Epub 2003 Nov 10.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-ENVYESIKEMGL
NCBI Gene ID #:
72699 (mouse); 362289 (rat);
NCBI Official Name:
Lck interacting transmembrane adaptor 1
NCBI Official Symbol:
Lime1
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_076173.1
Protein GI Number:
12963783
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology
SPECIFICITY:
.
Swissprot #:
Q9EQR5