IL-17 Antibody

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ProSci
Product Code: PSI-XP-5172
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5172-0.1mg0.1mg£524.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
IL-17 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

Images

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To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IL-17A (XP-5172Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.
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To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml - 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
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To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IL-17A (XP-5172Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.
To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml - 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

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Further Information

Additional Names:
IL17, CTLA8, IL-17, IL-17A, IL17, Interleukin-17A, Cytotoxic T-lymphocyte-associated antigen 8
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-17A (50.00 ng/mL), a concentration of 0.43 - 0.65 μg/mL of this antibody is required.

ELISA:

To detect hIL-17A by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.

Sandwich:

To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-17A (XP-5171Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.

Western Blot:

To detect hIL-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-17 (human Interleukin-17).
NCBI Gene ID #:
3605
NCBI Official Name:
interleukin 17A
NCBI Official Symbol:
IL17A
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q16552
Protein GI Number:
2498481
Purification:
Anti-hIL-17 specific antibody was purified by affinity chromatography employing immobilized hIL-17 matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
Q16552
User NOte:
Centrifuge vial prior to opening.